Command Reference

ChromoSort exposes one top-level command, chromo, with nine subcommands. Each subcommand page includes the purpose, examples, outputs, key parameters, and reasoning behind the command.

Command Use it when you need to
chromo sort assign contigs to reference sequences, filter duplicate overlaps, and write an ordered FASTA.
chromo clean sort-filter raw contigs, conservatively fix retained contigs, and write a cleaned FASTA.
chromo eval prepare editable TSV review tables for command-line or spreadsheet-first fix, scaffold, and gapfill curation, with optional graph and long-read evidence.
chromo fix split reviewed or automatically detected chimeric contigs into reference-labeled pieces.
chromo cut cut contigs at exact reviewed coordinates.
chromo manual generate a browser dashboard for manual dot-plot review, task-specific review-event queues, modular evidence panels, and reproducible recipe export. See the dot-plot guide if the visual patterns are unfamiliar.
chromo plot draw whole-genome, per-reference, or selected-reference dot plots from existing MUMmer coords or minimap2 PAF alignments. Use the dot-plot guide to interpret the patterns.
chromo scaffold join final sorted contigs into one scaffold per reference sequence, with optional reviewed gap overrides and report-only graph junction evidence.
chromo gapfill plan and optionally apply reviewed graph-supported fills between adjacent sorted contigs using guarded GFA paths and optional support evidence.